Mass Spectrometry of Peptides

Tandem Mass Spectrometry of Peptides

Renata Soares1, Elisabete Pires1, André M. Almeida1,2, Romana Santos1,3, Ricardo Gomes1, Kamila Koči1, Catarina Ferraz Franco1 and Ana Varela Coelho1 1ITQB/Universidade Nova de Lisboa 2Instituto de Investigação Cientifica Tropical& Centro Interdisciplinar de Investigação em Sanidade Animal 3Unidade de Investigação em Ciências Orais e Biomédicas, Faculdade de Medicina Dentária/Universidade de Li...

Mass spectrometry-based discovery of circadian peptides.

A significant challenge to understanding dynamic and heterogeneous brain systems lies in the chemical complexity of secreted intercellular messengers that change rapidly with space and time. Two solid-phase extraction collection strategies are presented that relate time and location of peptide release with mass spectrometric characterization. Here, complex suites of peptide-based cell-to-cell s...

Mass spectrometry of peptides and proteins.

This tutorial article introduces mass spectrometry (MS) for peptide fragmentation and protein identification. The current approaches being used for protein identification include top-down and bottom-up sequencing. Top-down sequencing, a relatively new approach that involves fragmenting intact proteins directly, is briefly introduced. Bottom-up sequencing, a traditional approach that fragments p...

Characterizing MHC-associated peptides by mass spectrometry.

Progress in plasma desorption mass spectrometry of peptides and proteins.

The principles and some applications of plasma desorption (PD) m.s. were described in this Journal in a report from the Biochemical Society's 618th Meeting in Liverpool on 2-4 April 1986 (Roepstorff et al., 1 9 8 7 ~ ) . Since then, PD-m.s. has developed to become a routine method in protein studies in our laboratory. During this period the sample preparation techniques using adsorption to nitr...